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. 2014 Mar 5;15(3):4007–4018. doi: 10.3390/ijms15034007

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Overexpression of miR-21 promotes NP cell proliferation. (A) Expression levels of miR-21 were examined using real-time PCR for non-transfected cells or after transfection of 50 nmol/L of a miR-21 mimic or a scramble control; (B) The growth of NP cells is shown for non-transfected cells or after transfection with 50 nmol/L of a miR-21 mimic or a scramble control. The growth index was assessed 1, 2, 3 and 4 days post-transfection; (C) miR-21 promotes cyclin D1 mRNA expression. NP cells were transfected with 50 nmol/L of a miR-21 mimic or a scramble control or remained non-transfected. Cyclin D1 was detected using real-time PCR; (D) miR-21 promotes cyclin D1 protein expression. NP cells were transfected with 50 nmol/L of a miR-21 mimic or a scramble control or remained non-transfected. Cyclin D1 was detected by western blot analysis. GAPDH was detected as a loading control. The values are presented as the mean ± SD. Compared with the control, * p < 0.05 and *** p < 0.001.