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. 2014 Mar 5;15(3):4007–4018. doi: 10.3390/ijms15034007

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — PTEN is a direct target of miR-21. (A) Predicted duplex formation between the human PTEN 3′UTR and miR-21. In the upper panel, the sequence alignment of miR-21 with the binding site of the PTEN 3′UTR is shown. In the lower panel, the sequence of the miR-10b binding site within the PTEN 3′UTR of three species is shown; (B) miR-21 does not alter the mRNA level of PTEN, as measured using real-time PCR; (C) The luciferase activity of wild-type (WT-UTR) or mutant (MUT-UTR) PTEN is shown; (D) PTEN protein expression in NP cells that were transfected with 50 nmol/L of a miR-21 mimic or a scramble control or remained non-transfected. The values are presented as the mean ± SD.