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. 2014 Mar 10;15(3):4201–4220. doi: 10.3390/ijms15034201

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — Significant enhancement of Caspase-3 activity caused by CE + DTX on B16 and MCF-7 cells (n = 3). Cells were treated with either CE (10 or 20 μM), DTX (20 or 40 μM), or CE + DTX (10 μM CE plus 20 μM DTX) for 24 h incubation, while 0.3% DMSO-PBS served as control. The Caspase-3 activity of cells with different treatments was evaluated by assessing the capacity to catalyze the cleavage of Caspase-3 substrate (Ac-DEVD-pNA) and release the pNA fluorochrome. (A) Caspase-3 activity of B16 cells; (B) Caspase-3 activity of MCF-7 cells. * p < 0.05, ** p < 0.01, statistically significant difference between CE and CE + DTX; ^## p < 0.01, statistically significant difference between DTX and CE + DTX.