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. 2014 Mar 11;15(3):4318–4332. doi: 10.3390/ijms15034318

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 1. — miR-124 regulates apoptosis in colorectal cancer cells. (A–D) DLD1, HCT116, SW480 or HT29 cells were transfected with miR-124 or antisense oligonucleotide (AS-miR-124), miR-Scr represented as the scrambled miRNA, and AS-Ctl represented the control of AS-miR-124 for 48 h. Apoptosis was assessed by Annexin V/PI assay; (E) miR-124 level was analyzed by real-time PCR, while HCT116 cells were transfected with miR-124 or AS-miR-124; (F,G) HCT116 cells were transfected with miR-124 or AS-miR-124, miR-Scr represented scrambled miR-124, AS-Ctl represented control of AS-miR-124 for 48 h; (F) Mitochondrial membrane potential was determined using JC-1. Loss of mitochondrial membrane potential was indicated by an increase in the green/red fluorescence intensity ratio. Fold-increase in green/red fluorescence was determined by comparing the results of treated samples with the level of the scrambled miRNA; (G) Caspase-9 activity or (H) Caspase-3 activity was analyzed by caspase-9 or caspase-3 Colorimetric Assay Kits respectively. Fold-increase in caspase-9 or caspase-3 activity was determined by comparing the results of treated samples with the level of the scrambled miRNA. The data are presented as mean ± SD of three independent experiments; (I) HCT-116 cells were seeded into six-well plates and treated with miR-124 or AS-miR-124. The fold-change of colony formation was determined by comparing the results of treated samples with the level of the scrambled miRNA. The data are presented as mean ± SD of three independent experiments. * p < 0.05 vs. control, ** p < 0.01 vs. control.

Figure 1. — miR-124 regulates apoptosis in colorectal cancer cells. (A–D) DLD1, HCT116, SW480 or HT29 cells were transfected with miR-124 or antisense oligonucleotide (AS-miR-124), miR-Scr represented as the scrambled miRNA, and AS-Ctl represented the control of AS-miR-124 for 48 h. Apoptosis was assessed by Annexin V/PI assay; (E) miR-124 level was analyzed by real-time PCR, while HCT116 cells were transfected with miR-124 or AS-miR-124; (F,G) HCT116 cells were transfected with miR-124 or AS-miR-124, miR-Scr represented scrambled miR-124, AS-Ctl represented control of AS-miR-124 for 48 h; (F) Mitochondrial membrane potential was determined using JC-1. Loss of mitochondrial membrane potential was indicated by an increase in the green/red fluorescence intensity ratio. Fold-increase in green/red fluorescence was determined by comparing the results of treated samples with the level of the scrambled miRNA; (G) Caspase-9 activity or (H) Caspase-3 activity was analyzed by caspase-9 or caspase-3 Colorimetric Assay Kits respectively. Fold-increase in caspase-9 or caspase-3 activity was determined by comparing the results of treated samples with the level of the scrambled miRNA. The data are presented as mean ± SD of three independent experiments; (I) HCT-116 cells were seeded into six-well plates and treated with miR-124 or AS-miR-124. The fold-change of colony formation was determined by comparing the results of treated samples with the level of the scrambled miRNA. The data are presented as mean ± SD of three independent experiments. * p < 0.05 vs. control, ** p < 0.01 vs. control.