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. 2014 Mar 12;15(3):4415–4430. doi: 10.3390/ijms15034415

Figure 4.

Figure 4.

Effect of carvacrol (CR) on C. difficile toxin regulatory genes. Brain heart infusion with or without the sub-inhibitory concentration (SIC) of CR, (0.60 mM) was inoculated (105 CFU/mL) separately with three hyper virulent C. difficile isolates, ATCC BAA 1870 (A); ATCC BAA 1053 (B) or ATCC BAA 1805 (C), and incubated anaerobically at 37 °C for 72 h. Bacterial pellets were harvested at 6 and 12 h for RNA isolation and RT-qPCR for toxin regulatory genes. * Treatments significantly differed from the controls (p < 0.05).