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. 2014 Mar 13;15(3):4442–4452. doi: 10.3390/ijms15034442

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 5. — Real-time PCR analysis of MC3T3-E1 cells grown in osteogenic media on Ti- or Zir-discs after 3, 7, and 10 day of culture. (A) Type I collagen; (B) Alkaline phosphatase (Alp); (C) Osteocalcin (Oc). Data are expressed as the mean ± SD of three independent experiments. Significance was tested by one-way ANOVA test. * Asterisks indicate p < 0.05 against the Ti-machined.