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. 2014 Mar 18;15(3):4733–4746. doi: 10.3390/ijms15034733

Figure 7.

Figure 7.

NOS activity (A); NO release (B) and baicalin content (C) in S. baicalensis cells irradiated with 20 kJ m−2 d−1 UV-B in the presence and absence of a NO scavenger, an NO donor and/or an NOS inhibitor. Five-day-old cultures were treated with 100 μmol L−1 of Nω-nitro-l-arginine (LNNA) (NOS inhibitor) with UV-B irradiation (UV-B + LNNA), 200 μM L−1 of cPTIO (NO scavenger) with UV-B irradiation (UV-B + cPTIO), 0.5 mM L−1 of sodium nitroprusside (SNP) (NO donor; SNP), 100 μM L−1 of LNNA and 0.5 mM L−1 of SNP with UV-B irradiation (UV-B + LNNA + SNP) or 0.5 mM L−1 of SNP and 200 μM L−1 of cPTIO (SNP + cPTIO). LNNA and cPTIO were added 30 min before UV-B treatment, and SNP was added at the same time as UV-B irradiation. The control cells were cultured in the dark and received the same volume of vehicle solvents only. Data are the means ± SE of three replicates.