Table 1.
Identification of phosphopeptides within HIP-55 protein over-expressed in HEK293 cells.
| Phosphopeptides | Experimental m/z (mono) | Theoretical m/z (mono) | Charge | XCorr | Position in protein |
|---|---|---|---|---|---|
| GACAS *HVSTMASFLK | 795.353 | 795.346 | 2 | 1.98 | 95–109 |
| GACAS *HVSTM *ASFLK | 803.351 | 803.344 | 2 | 1.94 | 95–109 |
| GAHVT *INAR | 509.744 | 509.745 | 2 | 2.37 | 110–118 |
| ASGANY *SFHKES *GR | 835.828 | 835.822 | 2 | 2.21 | 135–148 |
| TNAVS *EIK | 471.224 | 471.221 | 2 | 3.06 | 165–172 |
| VGKDS *FWAK | 559.253 | 559.258 | 2 | 2.57 | 174–182 |
| REQRY *QEQGGEAS *PQR | 1039.924 | 1039.926 | 2 | 1.94 | 220–235 |
| AMS *TTSISSPQPGK | 736.333 | 736.329 | 2 | 3.63 | 267–280 |
| AMS *TT *SISSPQPGK | 776.318 | 776.312 | 2 | 2.56 | 267–280 |
| AM *S *TTSISSPQPGK | 744.324 | 744.326 | 2 | 3.23 | 267–280 |
| LRS *PFLQK | 534.785 | 534.784 | 2 | 2.41 | 281–288 |
| QLT *QPETHFGR | 697.321 | 697.317 | 2 | 2.01 | 289–299 |
| QLT *QPET *HFGR | 737.304 | 737.300 | 2 | 1.98 | 289–299 |
| EPAAAIS *RPR | 574.289 | 574.285 | 2 | 2.49 | 300–309 |
NOTE: T *, S * and Y * refer to the phosphorylated form of threonine (Thr, T), serine (Ser, S) and tyrosine (Tyr, Y), respectively; M * refers to the oxidized methionine (Met, M).