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. 2014 Mar 20;15(3):5011–5031. doi: 10.3390/ijms15035011

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 7. — Effects of the forced expression of the Frizzled receptor FZD7 on gene expression and apoptosis in testicular cells and iPSCs. (a) Protein expression of FZD7 in bovine iPSCs and testicular cells transfected with pGK-CAS-FZD7 (200 and 400 ng) were examined. After cultivation of transfectants for 24 h, MEHP (10⁻⁴ and 10⁻⁵ M) were added and cultured for another 24 h and then subjected to Western blotting, as described in the Experimental Section; (b) pGK-CAS-FZD7 (400 ng) or each control vector pGK-CAS (400 ng) was introduced into bovine iPSCs, which were cultured for 24 h; 10⁻⁴ M MEHP was added and the cells cultured for another 24 h. The relative expression of AR was quantified by quantitative polymerase chain reaction (qPCR), as described in the Experimental section; (c) Effect of FZD7 on apoptosis. Apoptotic cells were quantified as described in the Experimental Section. Values measured with DMSO were defined as 1. Values indicated are means ± SEM, n ≥ 3; ^* p < 0.05, ^** p < 0.01.

Figure 7. — Effects of the forced expression of the Frizzled receptor FZD7 on gene expression and apoptosis in testicular cells and iPSCs. (a) Protein expression of FZD7 in bovine iPSCs and testicular cells transfected with pGK-CAS-FZD7 (200 and 400 ng) were examined. After cultivation of transfectants for 24 h, MEHP (10⁻⁴ and 10⁻⁵ M) were added and cultured for another 24 h and then subjected to Western blotting, as described in the Experimental Section; (b) pGK-CAS-FZD7 (400 ng) or each control vector pGK-CAS (400 ng) was introduced into bovine iPSCs, which were cultured for 24 h; 10⁻⁴ M MEHP was added and the cells cultured for another 24 h. The relative expression of AR was quantified by quantitative polymerase chain reaction (qPCR), as described in the Experimental section; (c) Effect of FZD7 on apoptosis. Apoptotic cells were quantified as described in the Experimental Section. Values measured with DMSO were defined as 1. Values indicated are means ± SEM, n ≥ 3; ^* p < 0.05, ^** p < 0.01.