Table 1.
Summary of the characteristics of stemness gene expression and pluripotency. The results of the immunocytochemical analysis of pluripotency and surface markers and RT-PCR analysis of transcripts of the “stemness” genes (encoding OCT4, SOX2, c-MYC, KLF4, STAT3, SUZ12, DNMT1, and MEF2A) in bovine testis cells and iPSCs are summarized.
| Characterizationof bovine testicular cells and induced pluripotent stem cells | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||
| Cell type | Teratoma formation | Cell-surface markers | Stem cell genes | |||||||
|
|
|
|||||||||
| SSEA-1 | SSEA-3 SSEA-4 |
AP | 4-Oct | Nanog | Sox2 | Klf4 | C-Myc | Stat3 | ||
| Testicular cells | − | +/− a | +/− a | +/− a | − | − | − | + | + | + |
| Testicular iPSCs | + | + | + | + | + | + | + | + | + | + |
a
The indicated cell type expressed each cell-surface marker weakly.
iPSCs: Induced pluripotent stem cells.