Table 2.
The PCR primers used in this study.
| Primer | Sequences (5′-3′) | Annotation |
|---|---|---|
| HSP1 | TCAGTCCAAAGCGACATCAAA | For 3′-RACE |
| HSP2 | CAGCCTACTTCAACGACTCAC | For 3′-RACE |
| Adaptor-R | AGCAGTGGTATCAACGCAGAG | Universal primers for 3′-RACE |
| Q2-F | CACAAACCCTAATTCTTTTTCATACA | Full length cloning |
| Q2-R | AGTAGAACAGATAAATATCGACCACA | Full length cloning |
| HSP-ORF-F2 | CcccgggATGGCTGGTAAAGGGTGAA | Open reading frame (ORF) cloning |
| HSP-ORF-R2 | GCtctagaGTCGACCTCTTCGATCTTGG | Open reading frame (ORF) cloning |
| CgHSP-Dra-F | AGGCtttaaaATGGCTGGTAAAGGTGA | Intracellular localisation of CgHSP70 |
| CgHSP-Not-R | GTgcggccgcGAGTCGACCTCT | Intracellular localisation of CgHSP70 |
| 1301-F | TCCcccgggGTTATGACGCTGGGAATGTTTT | pCAMBIA1301 vector modified |
| 1301-R1 | GCtctagaAGATAATGCCACAGCACCTCTT | pCAMBIA1301 vector modified |
| 1301-R2 | cGAGCTCtctagaAGATAATGCCACAGCACCTCTT | pCAMBIA1301 vector modified |
| GAPDH-F | GCTGTATCCCCATTCGTT | qRT-PCR |
| GAPDH-R | AGAAGGCAAGCTCAAGGG | qRT-PCR |
| HSP-F | GCTTGCTGAGGCTGATGAGT | qRT-PCR |
| HSP-R | ACCTGATGGTGCGGGTTCCTC | qRT-PCR |
| HptII-F | CTCGATGAG CTGATGCTTTGGG | verifying positive transformants |
| HptII-R | GCTTCTGCGGGCGATTTGTGTA | Verifying positive transformants |