Fig. 6.

Effect of snake venom activated NK-92MI cells on the activation of NF-κB in lung cancer cells (A549 and NCI-H460). Nuclear extract from A549 and NCI-H460 lung cancer cells co-administered with snake venom and NK-92MI cells for 1h was incubated in binding reactions of ³²P-end-labeled oligonucleotide containing the κB sequence. The activation of NF-κB was investigated using EMSA as described in Materials and Methods (A). The NF-κB binding activity was confirmed with supershift assay with anti p50 antibody (0.5 μg) (B).