Fig. 5.

Effect of apigenin on phorbol ester-induced increases in phospho-ERK1/2 levels. Phospho-ERK1/2 protein levels were decreased in quick frozen apigenin-treated rat aortas in the absence of endothelium compared to vehicle-treated rat aortas precontracted with phorbol ester. The upper panel shows a typical blot and the lower panel shows average densitometry results for relative levels of phospho-ERK1/2. Data are expressed as the means of 3–5 experiments with vertical lines representing SEMs. ^*p<0.05, ^##p<0.01, versus control or normal group respectively. Apigenin: 0.1 mM apigenin; PDBu: 1 μM phorbol 12,13-dibutyrate.