Figure 5.

Src is activated in L929 cells by TNF stimulation in a Gγ10-dependent but necrosome-independent manner. (A) Control and Gγ10-knockdown L929 cells were treated with TNF (10 ng/ml) for the indicated time periods. Cells were then harvested and the expression levels of the total Src kinase and Tyr418-phosphorylated Src in whole-cell extract were examined by immunoblotting with anti-Src and anti-phospho-Tyr418-Src antibodies. (B-D) The same as in A except that control and RIP1-knockdown, RIP3-knockout, or TNFR1-knockdown L929 cells were used. See also Supplementary information, Figure S3.