Figure 6. Centromeres coincide with transcription factor hotspots.
(A) MEME motif for the 80-bp cores of centromeric sites (left) and the 80-bp cores of high occupancy target (HOT) sites (right). (B) Heatmaps and average plots of cenH3 ChIP, insoluble chromatin and CENP-C ChIP signal within a 2-kb window around HOT sites, illustrating that HOT sites are highly occupied by cenH3, insoluble chromatin and CENP-C. Each line of the heatmaps represents an individual HOT site. Heatmaps are sorted by decreasing signal.
Figure 6—figure supplement 1. CenH3 site motif and characterization of HOT sites.
(A) MEME motif for 80-bp cores of all centromeric sites. (B) Venn diagram illustrating the overlap between cenH3 sites and HOT sites. Hypergeometric p=8.6 × 10−161. (C) Average signal of nucleosome-size input MNase fragments (141–500 bp) within a 2-kb window around all 248 HOT sites. (C) Average cenH3 ChIP signal within a 2-kb window around all 248 HOT sites in wildtype and knl-2(RNAi) embryos. Differences between ChIP and input are plotted. (D) Heatmap of difference in cenH3 ChIP signal between wildtype and knl-2(RNAi) at all 248 HOT sites. Each line of the heatmap represents an individual HOT site. The heatmap is sorted by decreasing difference between wildtype and knl-2(RNAi).