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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Biomaterials. 2014 Feb 21;35(14):4382–4389. doi: 10.1016/j.biomaterials.2014.01.078

Fig. 6.

Fig. 6

Effect of protein type and salt concentration on protein-chitosan binding. Isothermal titration calorimetry of ovalbumin (OVA) and bovine serum albumin (BSA) into chitosan indicates differences in reactions dependent upon the protein and salt concentration. The top panel of each figure shows the raw heats per injection with each peak representing a single injection. The lower panel shows the integrated total enthalpy per mole of injectant as a function of the molar ratio of the protein to the chitosan with each point representing a single injection. BSA or OVA solutions were titrated into chitosan in deionized water, 10 or 25mM NaCl as indicated. All titrations were performed at a pH of 6.5 and 25 °C using Microcal’s VP-ITC with 100 nM chitosan in the cell and 40 M protein in the syringe.