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Experimental design for protein release studies: Effect of particle size
| Nominal size |
Chitosan conc. |
Precipitant salt |
Protein | Salt addition rate |
Actual Mean Size (nm) |
Protein loading efficiency (%) |
|---|---|---|---|---|---|---|
| 300nm | 0.5 mg/ml | Na2SO4 | FITC-BSA | 8 ml/min | 318.2±11 | 58.1±3.7 |
| 1μm | 3 mg/ml | Na2SO4 | FITC-BSA | 1 ml/min | 1133±54.1 | 83.5±8.3 |
| 3μm | 5 mg/ml | Na2SO4 | FITC-BSA | 0.2 ml/min | 2871±216 | 96.6±0.6 |
