Table 2.
Species identification results obtained upon phenotypic testing and molecular typing of 35 Malassezia cultures
| Strain no. |
Species identification by means of |
Final species identification d | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Phenotypic methods a |
Molecular methods |
|||||||||
| PCR-RFLP b |
PCR sequencing
c
|
|||||||||
| ITS accession no. | Similarity with ITS of [%]: | D1/D2 accession no. | Similarity with D1/D2 of [%]: | |||||||
|
1. |
40.10.I. |
M. slooffiae |
M. sympodialis |
KC141968 |
M. sympodialis MA 73 |
100% |
KC415092 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
2. |
40.10.II. |
M. slooffiae |
M. sympodialis |
KC141969 |
M. sympodialis CBS 7222 |
100% |
KC415093 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
3. |
40.10.IV. |
M. sympodialis |
M. sympodialis |
KC141970 |
M. sympodialis CBS 7222 |
100% |
KC415094 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
4. |
8.11.I. |
M. obtusa |
M. sympodialis |
KC152895 |
M. sympodialis CBS 7222 |
100% |
KC415085 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
5. |
8.11.III. |
M. furfur |
M. sympodialis |
KC152904 |
C. diffluens CBS 160 |
100% |
KC241877 |
C. diffluens CBS 6436 |
100% |
M. sympodialis + C. d.
*
|
|
6. |
8.11.IV. |
M. sympodialis |
M. sympodialis |
KC152896 |
M. sympodialis CBS 7222 |
100% |
KC415086 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
7. |
27.10.III. |
M. sympodialis |
M. sympodialis |
KC141966 |
M. sympodialis MA 73 |
100% |
KC415095 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
8. |
27.10.IV. |
M. sympodialis |
M. sympodialis |
KC141967 |
M. sympodialis MA 73 |
100% |
KC415096 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
9. |
7.11.III. |
M. sympodialis |
M. sympodialis |
KC152893 |
M. sympodialis CBS 7222 |
100% |
KC415083 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
10. |
7.11.IV. |
M. sympodialis |
M. sympodialis |
KC152894 |
M. sympodialis MA 73 |
100% |
KC415084 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
11. |
25.10.IV. |
M. sympodialis |
M. sympodialis |
KC119577 |
M. sympodialis CBS 7222 |
100% |
KC415097 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
12. |
17.10.IV. |
M. sympodialis |
M. sympodialis |
KC119576 |
M. sympodialis CBS 7222 |
100% |
KC415098 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
13. |
10.11.I. |
M. sympodialis |
M. sympodialis |
KC152897 |
M. sympodialis CBS 7222 |
100% |
KC415087 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
14. |
10.11.II. |
M. sympodialis |
M. sympodialis |
KC152905 |
A. pullulans CPC 13701 |
100% |
KC241878 |
A. pullulans RA406 |
100% |
M. sympodialis + A. p.** |
|
15. |
10.11.IIIA. |
M. furfur |
M. furfur |
KC152897 |
M. furfur M235 |
100% |
KC415088 |
M. furfur VG Ig 02 |
99% |
M. furfur |
|
16. |
10.11.IV. |
M. sympodialis |
M. sympodialis |
KC152901 |
M. sympodialis CBS 7222 |
100% |
KC415091 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
17. |
45.08.II. |
M. furfur |
M. furfur |
KC141972 |
M. furfur M235 |
100% |
KC415099 |
M. furfur VG Ig 02 |
100% |
M. furfur |
|
18. |
45.08.III. |
M. furfur |
M. furfur |
KC141973 |
M. sympodialis CBS 7222 |
100% |
KC415100 |
M. sympodialis IFM 48109 |
99% |
M. furfur + M. sympodialis |
|
19. |
20.09.IV. |
M. furfur |
M. furfur + M. sympodialis |
KC141965 |
M furfur M235 |
100% |
KC415101 |
M. furfur VG Ig 02 |
99% |
M. furfur + M. sympodialis |
|
20. |
17.09.IV. |
M. sympodialis |
M. sympodialis |
KC109788 |
M. sympodialis MA 73 |
100% |
KC415102 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
21. |
43.08.II. |
M. furfur |
M. furfur |
KC141971 |
M. furfur M235 |
100% |
KC415103 |
M. furfur VG Ig 02 |
100% |
M. furfur |
|
22. |
6.11.IV. |
M. sympodialis |
M. sympodialis |
KC152892 |
M. sympodialis MA 73 |
100% |
KC415082 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
23. |
1.11.IA. |
M. globosa |
M. globosa |
KC152884 |
M. globosa CBS 7966 |
96% |
KC415074 |
M. sympodialis IFM 48109 |
100% |
M. globosa |
|
24. |
1.11.II. |
M. globosa |
M. globosa + M. restricta |
KC152885 |
M. restricta MRE28 |
99% |
KC415075 |
M. sympodialis IFM 48109 |
100% |
M. globosa + M. restricta |
|
25. |
1.11.IIIA. |
M. sympodialis |
M. sympodialis |
KC152886 |
M. sympodialis MA 73 |
100% |
KC415076 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
26. |
1.11.IVA. |
M. furfur |
M. sympodialis |
KC152888 |
M. sympodialis MA 73 |
100% |
KC415078 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
27. |
69.09.III. |
M. slooffiae |
M. slooffiae |
KC141978 |
M. slooffiae CBS 7956 |
99% |
KC415104 |
M. slooffiae sc7LG |
99% |
M. slooffiae |
|
28. |
69.09.IV. |
M. slooffiae |
M. slooffiae |
KC141979 |
M. slooffiae CBS 7956 |
99% |
KC415105 |
M. sympodialis IFM 48109 |
100% |
M. slooffiae + M. sympodialis |
|
29. |
67.09.III. |
M. sympodialis |
M. sympodialis |
KC141974 |
M. sympodialis CBS 7222 |
100% |
KC415106 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
30. |
67.09.IV. |
M. slooffiae |
M. sympodialis |
KC141975 |
M. sympodialis CBS 7222 |
100% |
KC415107 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
31. |
68.09.III. |
M. sympodialis |
M. sympodialis |
KC141976 |
M. sympodialis CBS 7222 |
100% |
KC415108 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
32. |
68.09.IV. |
M. sympodialis |
M. sympodialis |
KC141977 |
M. sympodialis CBS 7222 |
100% |
KC415109 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
33. |
2.11.III. |
M. sympodialis |
M. sympodialis |
KC152890 |
M. sympodialis MA 73 |
100% |
KC415080 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
|
34. |
2.11.IV. |
M. obtusa |
M. sympodialis |
KC152891 |
M. sympodialis MA 73 |
100% |
KC415081 |
M. sympodialis IFM 48109 |
100% |
M. sympodialis |
| 35. | 12.09.IV. | M. sympodialis | M. sympodialis | JX915741 | M. sympodialis MA 73 | 100% | KC415110 | M. sympodialis IFM 48109 | 100% | M. sympodialis |
a Identification algorithm included macro- and microscopic examination of fungal colonies along with biochemical tests, as described in the Materials and Methods section.
b Based on two PCR-RFLP assays (i. e. with AluI and HaeII restriction endonucleases).
c Results of BLAST search of GenBank (http://blast.ncbi.nlm.nih.gov/); The ITS and D1/D2 accession numbers are those under which the respective sequences determined in this study can be found in the GenBank; Percentage similarities with ITS or D1/D2 sequences deposited in the GenBank are given.
d Based on combined molecular methods results; *C. d., Cryptococcus diffluens; **A. p., Aureobasidium pullulans.