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. Author manuscript; available in PMC: 2014 Apr 4.
Published in final edited form as: Mol Cancer Res. 2013 Jul 15;11(10):1223–1234. doi: 10.1158/1541-7786.MCR-13-0252-T

Fig. 3.

Fig. 3

Effect of 53BP1 deficiency on the ATM/p53 axis function. A, thymocytes were irradiated in vitro and harvested at the indicated timepoints for immunoblotting. Total KAP1 serves as loading control. B, mice were irradiated, allowed to repair in vivo and euthanized at the indicated timepoints. Thymocytes were analyzed as in A. C, Trp53bp1−/−/Atm−/− thymocytes were irradiated in the presence or absence of the DNA-PKcs inhibitor NU7026 and harvested at 3 hours for immunoblotting. D–F, mice were irradiated and euthanized at either 3 or 6 hours post-IR. D, thymus RNA was reverse transcribed and p21 or Bax cDNA amplified by Real-Time RT-PCR. E–F, apoptosis was quantified in thymic sections using the TUNEL assay. Representative examples are shown in F. Bars represent the average and standard deviation of 3 experiments.