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Mitochondria isolated from wild type and a strain expressing Mic10-His6 were lysed with 1% digitonin or 1% TX-100 and incubated with NiNTA-beads. Bound proteins were eluted using Laemmli buffer containing 300 mM imidazole. Total (T), (5%); supernatant (S), (5%); and bound material (B), (100%) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. Aco1, aconitase.
