Figure 4. Combination of AIM24 deletion with His-tagging of Mic12 and Mic26, but not of Mic19 and Mic27, leads to respiratory deficiency and instability of the MICOS complex.
(A) Wild type and Δaim24 cells were grown in YPD, YPG or lactate liquid medium at 30°C to logarithmic phase. The cultures were diluted in the respective medium to OD600 of 0.2 and further incubated at 30°C. Aliquots were taken every 2 hr and OD600 was measured. Black, wild type; grey, Δaim24. (B) Growth of the indicated strains on agar plates containing YPD or lactate medium. Cells of the indicated strains were grown in YPD liquid medium at 30°C to logarithmic phase. Serial dilution was performed, 3 µl of each dilution was spotted on the indicated medium and incubated at 30°C. (C) Molecular sizing chromatography on Superose 6 column of the indicated strains and immunodetection of the indicated MICOS subunits. Analysis was performed as described in Figure 2A–E. Asterisks indicate a cross reaction of the Mic60 antibody used in this analysis.