Figure 5. Deficiency of respiratory growth of Δaim24 mutants are not caused by lack of mitochondrial DNA, but by lack of OXPHOS supercomplexes and of a selective set of proteins involved in ATP production and transport.
(A) Fluorescence images of DAPI stained cells of the indicated strains. Scale bar, 5 µm (left panel). Quantitative analysis of levels of mitochondrial DNA by DAPI staining (right panel). (B) Analysis of respiratory supercomplexes of isolated mitochondria by Blue native gel electrophoresis and immunoblotting with antibodies against cytochrome c1 (Cyt1). The positions of supercomplexes consisting of complex III (III2) and of complexes III plus IV (III2IV and III2IV2) are indicated. (C) Steady state levels of proteins of mitochondria isolated from the indicated cells grown in the presence of lactate medium containing 0.1% glucose.