Figure 6. Cells lacking Aim24 and containing Mic12-His6 or Mic26-His6 are deficient in remodelling of cardiolipin and are deficient in tafazzin.
(A) Acyl chain distribution of cardiolipin. First numbers represent the sum of the carbon atoms in the four acyl chains; the numbers behind the colon indicate the total number of double bonds. Abbrevations of strains as in Figure 5. (B) Tafazzin (Taz1) is strongly down regulated in the absence of Aim24 and presence of His-tagged Mic12 or Mic26. Isolated mitochondria were analyzed by SDS-PAGE and immunodetection of the indicated proteins. (C) Presence of Taz1 is not dependent on the presence of Mic60 and Mic10. Isolated mitochondria were analyzed by SDS-PAGE and immunodetection of the indicated proteins.