Table 4.
Strain | Genotype |
---|---|
Δaim24 | MATα, ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, aim24::HIS3 |
Aim24-His6 | MATα, ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, Aim24-His6-KAN |
Aim24-3xHA | MATα, ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, Aim24-3xHA- HIS3 |
Δaim24 Mic10-3xHA | MATα ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, aim24::HIS3, Mic10-3xHA-KAN |
Δaim24 Mic12-His6 | MATα, ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, aim24::HIS3, Mic12-His6-KAN |
Δaim24 Mic19-His6 | MATα, ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, aim24::HIS3, Mic19-His6-KAN |
Δaim24 Mic27-His6 | MATα, ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, aim24::HIS3, Mic27-His6-KAN |
Δaim24 Mic26-His6 | MATα, ura3-52, lys2-801amber, ade2-101ocre, trp1-Δ63, his3-Δ200, leu2-Δ1, aim24::HIS3, Mic26-His6-KAN |
All chromosomal manipulations were performed by homologous recombination using the indicated marker cassettes. Left column, names of the various strains; right column, genotypes of the respective strains.