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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Cancer Res. 2014 Feb 3;74(7):2050ā€“2061. doi: 10.1158/0008-5472.CAN-13-1659

Figure 5.

Figure 5

Effect of CBP and PTEN deficiency on expression of total and T350-phosphorylated EZH2, H3K27Ac and H3K27me3 levels on bulk histones. A, WB analysis of indicated proteins in Pten-proficient (+/+) and -deficient (āˆ’/āˆ’) MEFs. B, DU145 cells transfected as indicated for 48 h followed by WB analysis. Cā€“E, DU145 cells were transfected as indicated for 36 h. Cytosolic (PTEN, ERK2) and nuclear (CBP, H3K27Ac, H3K27me3 and H3) extracts were prepared for western blot (chemiluminescence-based) analysis of expression of indicated proteins (C). The relative expression of H3K27Ac and H3K27me3 was determined by normalizing to histone H3 first and then to the normalized value in siC-transfected cells and the quantitative data are shown in (D) and (E), respectively. The experiments were repeated twice. Similar results were obtained from these experiments and the data shown was from one experiment.