Table 1.
List of murine proteins identified by MS as binding partners of the IQGAP1 Repeats.
| Protein name | Gene name | Accession number | High Confidence Peptides | Percent Sequence Coverage | Subcellular localization | Function |
|---|---|---|---|---|---|---|
| Ezrin | Ezr | P26040 | 5 | 38 | C,CM,CSK | Actin filament binding |
| Radixin | Rdx | P26043 | 4 | 24 | C,CM,CSK | Actin capping |
| Moesin | Msn | P26041 | 6 | 31 | C,CM,CSK | Cytoskeleton related (inferred) |
| F-actin-capping protein subunit α2 | Capza2 | P47754 | 1 | 10 | C,CM,CSK1 | Actin capping |
| β-centractin | Actr1b | Q8R5C5 | 5 | 28 | C,CM | ATP binding |
| Rho GDI | Arhgdia | Q99PT1 | 5 | 41 | C | GTPase activation |
| G protein subunit β1 | Gnb1 | P62874 | 2 | 13 | CM1 | Signal transducer |
| Aspartoacylase-2 | Acy3 | Q91XE4 | 6 | 39 | C,CM | Hydrolase |
| α-enolase/MBP-1 | Eno1 | P17182 | 9 | 51 | C,N | Phosphopyruvate hydratase/Mg2+ binding |
| Purine nucleoside phosphorylase | Pnp | P23492 | 9 | 37 | C | Glycosyltransferase |
| Eukaryotic initiation factor 4A-II | Eif4a2 | P10630 | 1 | 24 | C2 | Helicase/hydrolase/initiation factor |
| Elongation factor 2 | Eef2 | P58252 | 1 | 6 | C | Elongation factor |
| Adenosylhomocysteinase(SAHH) | Ahcy | P50247 | 9 | 33 | C | Hydrolase |
| 10-FTHFDH | Aldh1l1 | Q8R0Y6 | 9 | 26 | C | Oxidoreductase |
| Transaldolase | Taldo1 | Q93092 | 3 | 33 | C | Transferase |
| Aldo-keto reductase family 1 member C21 | Akr1c21 | Q91WR5 | 4 | 27 | C | Oxidoreductase |
| Homogentisicase | Hgd | O09173 | 5 | 15 | C2 | Oxidoreductase/dioxygenase |
| Peroxiredoxin-1 | Prdx1 | P35700 | 7 | 58 | C | Antioxidant/oxidoreductase/Peroxidase |
| Peroxiredoxin-2 | Prdx2 | Q61171 | 3 | 37 | C | Antioxidant/oxidoreductase/peroxidase |
| λ-crystallin homolog | Cryl1 | Q99KP3 | 4 | 28 | C | Oxidoreducatse |
| Inorganic pyrophosphatase | Ppa1 | Q9D819 | 3 | 34 | C | Hydrolase |
| ω-amidase | Nit2 | Q9JHW2 | 2 | 12 | C | Hydrolase |
| Bifunctional ATP-dependent dihydroxyacetone kinase /FAD-AMP lyase (cyclizing) | Dak | Q8VC30 | 7 | 23 | C2 | Kinase/lyase/transferase |
| Sedoheptulokinase | Shpk | Q9D5J6 | 2 | 5 | C | Kinase/transferase |
| abhydrolase domain-containing protein 14B | Abhd14b | Q8VCR7 | 4 | 40 | C,N | Hydrolase |
| Fructose-1,6-bisphosphatase1 | Fbp1 | Q9QXD6 | 9 | 50 | C1 | Hydrolase |
| 26S protease regulatory subunit 7 | Psmc2 | P46471 | 6 | 28 | C,N | ATP-binding/ATPase |
| Antiquitin-1 | Aldh7a1 | Q9DBF1 | 3 | 19 | C,M,N | Oxidoreductase |
| Sorting nexin 1 | Snx1 | Q9WV80 | 2 | 6 | C,CM,VM,G | Phosphatidylinositol binding/protein transporter |
| 78 kDa glucose-regulated protein | Hspa5 | P20029 | 2 | 11 | C,ER | ATP binding/misfolded protein binding/ribosome binding |
| Voltage-dependent anion-selective channel protein 1 | Vdac1 | Q60932 | 4 | 33 | CM,MM | Porin |
| V-type proton ATPase subunit B, brain isoform | Atp6v1b2 | P62814 | 8 | 32 | C,CM,VM,G | ATP binding/ hydrolase/transmembrane transport |
| Catalase | Cat | P24270 | 4 | 25 | P | NADP binding/catalase activity |
| Aconitate hydratase | Aco2 | Q99KI0 | 6 | 22 | M | Iron,sulfur cluster binding/hydratase |
| Cytochrome b-c1 complex subunit 1 | Uqcrc1 | Q9CZ13 | 9 | 42 | MM | Catalytic/metal ion binding |
| Peroxiredoxin-3 | Prdx3 | P20108 | 2 | 19 | M | Peroxidase |
| Pyruvate dehydrogenase E1 component subunit β | Pdhb | Q9D051 | 7 | 34 | M | Oxidoreductase |
| 2-oxoglutarate dehydrogenase | Ogdh | Q60597 | 3 | 17 | M | Oxidoreductase |
| Dimethylargininase-1 | Ddah1 | Q9CWS0 | 6 | 45 | M1 | Hydrolase |
| Heterogenous nuclear riboprotein D-like | Hnrpdl | Q9Z130 | 1 | 8 | C,N | Transcription |
| Serotransferrin | Tf | Q921I1 | 8 | 34 | S | Iron transport |
| Fibrinogen β chain | Fgb | Q8K0E8 | 2 | 15 | S | Eukaryotic cell surface binding |
1, information from neXtProt; NA: not available; C: cytoplasm; CM: cell membrane; CSK: cytoskeleton; ER: endoplasmic reticulum; G: golgi; MM: mitochondria membrane; M: mitochondrion; N: nucleus; S: secreted; P: peroxisome; VM: vacuole membranes