Figure 1.
Designs of the promoter constructs and comparisons of luciferase activity among macrophage-specific SPs. (a) Constructs of SPs. SP107 and SP146 were inserted in front of p47phox elements containing the PU.1 site (C1, C2 and C3). All promoters were constructed on the pGL3-basic plasmid. (b) The non-macrophage cell lines 293 T and HeLa and the macrophage cell line RAW264.7 were transfected, and promoter activities were measured 48 h later by dual-luciferase assay. Renilla luciferase vector was co-transfected with SPs for control of the transfection efficiency. Luciferase activity was further normalized to that obtained with the pGL3-basic vector. (c) To evaluate macrophage specificity, promoter activities were represented as the ratios of RAW264.7/293 T and RAW264.7/HeLa. Data shown are means±s.d.'s.