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. 2014 Feb 6;21(4):353–362. doi: 10.1038/gt.2014.3

Figure 4.

Figure 4

Constructs of lentiviral vectors for APN expression. (a) Macrophage-specific lentiviral vectors were generated. (b) Cell-type-specific GFP expression in 293 T, RAW264.7 and THP1 cells infected by macrophage-specific lentivirus GFP (Lenti-GFP) and SP146-C1-GFP (Lenti-SP-GFP) lentivirus. GFP-expressing cells were distinguished from lentivirus-infected cells under fluorescence microscopy at × 200 magnification. (c) GFP-positive cells were counted and the ratio of the cell number in Lenti-SP-GFP-infected group to Lenti-GFP-infected group (SP-GFP/GFP) is shown. (d) The expressions of GFP mRNA were analyzed by RT-PCR, and the relative densities of the bands are shown. Higher expression ratio of Lenti-SP-GFP to Lenti-GFP was observed in RAW264.7 and THP1 cells than in 293 T cells. Data shown are means±s.d.'s. (e) Macrophage specificity of lentiviral-GFP was tested in bone marrow-derived mononuclear cells (BM-MNC) and bone marrow-derived macrophages (BMDM). Representative images were shown, and the ratio of mRNA and Luc activity were expressed in graphs. *P<0.05 and **P<0.01. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Luc, luciferase.