Table 2. Thermodynamic parameters of (GlcNAc)_3–6 binding to the mutant E148Q derived from ITC.

	n	K d (µM)	ΔG (kcal mol−1)	ΔH (kcal mol−1)	−TΔS (kcal mol−1)
(GlcNAc)3	1.84 ± 0.12	K d1, 76.9 ± 18.9; K d2, 284.1 ± 97.4	ΔG 1, −5.70; ΔG 2, −4.91	ΔH 1 −1.47 ± 0.15; ΔH 2, −1.21 ± 0.34	−TΔS 1, −4.23; −TΔS 2, −3.70
(GlcNAc)4	0.93 ± 0.01	11.3 ± 1.5	−6.84	−2.17 ± 0.05	−4.67
(GlcNAc)5	0.92 ± 0.02	2.0 ± 0.2	−7.89	−2.89 ± 0.23	−5.00
(GlcNAc)6	0.93 ± 0.02	0.48 ± 0.09	−8.76	−1.45 ± 0.02	−7.31