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. 2014 Mar 19;70(Pt 4):981–993. doi: 10.1107/S1399004713034718

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© Torreira et al. 2014

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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Nano-electrospray mass spectra of M. truncatula GSII-2a. (a) MS spectrum acquired for the intact MtGSII-2a assembly with a measured mass of 441 814 ± 75 Da, which is in good agreement with it existing as a decamer composed of ten copies of the M. truncatula GSII-2a monomer (b). (c) The spectrum recorded under harsh MS conditions is dominated by two charge-state series corresponding to the M. truncatula GSII-2a decamer (series centred at 41+) and pentamer (series centred at 27+), respectively, suggesting that the intact complex consists of two labile interacting pentamers. (d) Expansion of the m/z region below an m/z of 2500 in (c) showing M. truncatula GSII-2a monomers dissociated both from pentameric and decameric complexes in the gas phase.