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Wnt5a does not alter the total expression and phosphorylation of β-catenin. MG-63 were untreated or treated with 100 ng/ml of Wnt5a and harvested at 15 min, 30 min, and 1 h after the start of treatment for SDS-PAGE and immunoblot analysis with antibodies to p-β-catenin (p-Ser33) and total β-catenin. The total expression and phosphorylation of β-catenin were not been altered by Wnt5a stimulation.
