Figure 3.

Transgenic expression of Eda increases number of plates and alters neuromast distribution. (A-E) Plates were visualized with calcein (green) and neuromasts were visualized with DASPEI (purple) on representative control and transgenic fish from PB and PB x JP low plated F3 backgrounds, the Ma/Mp boundary is shown (white dotted line); schematics below each image show plate edges (gray lines), neuromast placement (black dots), Ma/Mp boundary (vertical or near-vertical gray dotted line) and the midline (blue dashed line). For all fish, dorsal is up and rostral is to the left, with the shoulder girdle (cleithrum) appearing to the left of the first Ma segment. The standard length of each fish is indicated in mm. (A) PB control; (B) PB transgenic; (C) PB x JP low plated F3 control; (D) PB x JP low plated F3 transgenic; (E) Remainder of the flank from the individual shown in (D). Individual frames comprising (B) were tilted slightly with respect to each other to correct for bend in fish body during photographing. DASPEI, 2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide; JP, Japanese Pacific Ocean marine; Ma, main trunk line, anterior; Mp, main trunk line, posterior; PB, Paxton benthic; PB x JP F3, Paxton benthic x Japanese Pacific Ocean marine F3 hybrid.