Table 1. Recombinant protein production information.

In the primers, the sequence given in upper-case letters denotes the insert-specific sequence, and the lower-case letters denote the overhang required for the SLIC vector. In the protein sequence, the coronin sequence is indicated in bold, and the 6×His tag and the 3C cleavage site are in italics. Thus, the final protein contains an additional GPG in the N terminus after proteolytic cleavage of the 6×His tag.

Source organism	T. gondii
DNA source	Synthetic (codon-optimized for insect cells)
Forward primer	5′-cag gga ccc ggt GCC GAC GCT GTA GAC G
Reverse primer	5′-cga gga gaa gcc cgg tta AGG TTT GAC ACT ACG TCT AC
Cloning vector	pBS II SK(+)
Expression vector	pFastBacNKI-his-3c-LIC
Expression host	Spodoptera frugiperda (Sf21)
Complete amino-acid sequence of the construct produced	MAHHHHHHSAALEVLFQGPG ADAVDVPLIKNLYAEAWKQQYSDLRLSTKQTESCGLAANTEYIAAPWDVGGGGVLGILRLADIGRNPAVAKIKGHTASIQDTNFSPFYRDILATACEDTIVRIWQLPEEVTGTTELKEPIATLTGALKKVLSAEWNPAVSGILASGCFDGTVAFWNVEKNENFASVKFQESLLSAKWSWKGDLLACTTKDKALNIVDPRAAQVVGSVACHDGSKACKCTWIDGLAGRDGHVFTTGFGKMQEREMAIWDTRKFDKPVYHAEIDRGSSPLYPIFDETTGMLYVCGKGDSSCRYYQYHGGTLRSVDAYRSSVPIKNFCFIPKLAVDQMRAEIGRMLKQENGNVLQPISFIVPRKNQDVFQADLYPPAPDVEPSMTAEEWFKGENKAIRRRSVKP