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. 2014 Mar 25;70(Pt 4):517–521. doi: 10.1107/S2053230X14005196

Table 2. Crystallization details of TgCorΔcc.

Method Sitting-drop vapour diffusion
Plate type Swissci MRC 2 96-well plates
Temperature (K) 295
Protein concentration (mg ml−1) 8
Buffer composition of protein solution 100 mM NaCl, 100 mM ammonium sulfate, 50 mM sodium phosphate buffer pH 6.5, 1 mM TCEP
Composition of reservoir solution 200 mM magnesium acetate, 25% PMME 2000, 100 mM Tris–HCl pH 7.5
Volume and ratio of drop 0.3 µl protein + 0.3 µl reservoir
Volume of reservoir (µl) 40