Table 3. Genes and amplicons used in the ‘all body fluids’ hexaplex HRM assay.
| Body fluid/Tissue | Gene | Amplicon T m | Primer Sequence (5′–3′) | |
|---|---|---|---|---|
| °C | Range (± 3SD) | |||
| Saliva | HTN3 | 76.6 | 76.0 – 77.2 | F: GCAAAGAGACATCATGGGTA
R: GCCAGTCAAACCTCCATAATC |
| Vaginal | IL19 | 78.6 | 78.0 – 79.2 | F: AACCACGGTCTCAGGAGATG
R: TGACATTTGGGAAGGTGTCC |
| Menstrual Blood | MMP10 | 81.9 | 81.5 – 82.3 | F:
GGGGGTGACGTTGGTCACTTCAGCTC
R: GGGGGCTGGAGAATGTGAGTGGAGT |
| Semen | TGM4 | 82.7 | 82.4 – 83.0 | F: ATGGTGTAAAGAGGACATGGTT
R: GGGAAATGCAGCAGTCCAG |
| Skin | CCL27 | 84.9 | 84.8 – 84.9 | F: AGCACTGCCTGCTGTACTCA
R: AGATGCTGCGTTGAGCCA |
| Blood | ALAS2 | 86.8 | 86.6 – 86.9 | F: TGTGTCCGTCTGGTGTAGTA
R: GAGTCATTGGCAACAAAGCA |
| DNA | -- | 80.6 | 80.2 – 80.9 | NA (product originates from IL19) |
*underlined text indicates non-template sequence addition