Table 1. Genes and amplicons used in singleplex, duplex and triplex body fluid HRM assays.
| Body fluid | Gene | Accession
number |
Amplicon size
(bp) |
Amplicon T m | Primer sequence (5′–3′) | |
|---|---|---|---|---|---|---|
| °C | Range (± 3SD) | |||||
| Vaginal | IL19 | NM_153758 | 189 | 81.5 | 81.3 – 81.7 | F: AACCACGGTCTCAGGAGATG
R: GAACGCCAGGAGGTTCTTG |
| Skin | IL1F7 | NM_014439 | 92 | 83.2 | 82.9 – 83.5 | F: CCAGTGCTGCTTAGAAGACC
R: TCACCTTTGGACTTGTGTGAA |
| Blood | ALAS2 | NM_001037967 | 136 | 85.8 | 85.6 – 86.0 | F: TGTGTCCGTCTGGTGTAGTA
R: AAACTTACTGGTGCCTGAGA |
| Menstrual blood | MMP10 | NM_002425 | 227 | 82.2,
83.5 |
81.8 – 82.7,
83.0 – 84.0 |
F: ACAGGGAAGCTAGACACTGA
R: CTGGAGAATGTGAGTGGAGT |
| Saliva | HTN3 | NM_000200 | 134 | 76.3 | 75.9 – 76.7 | F: GCAAAGAGACATCATGGGTA
R: GCCAGTCAAACCTCCATAATC |
| Semen | TGM4 | NM_003241 | 164 | 82.3 | 82.2 – 82.4 | F: ATGGTGTAAAGAGGACATGGTT
R: GGGAAATGCAGCAGTCCAG |