. 2014 Feb 26;2:281. Originally published 2013 Dec 20. [Version 2] doi: 10.12688/f1000research.2-281.v2

Table 3. Genes and amplicons used in the hexaplex body fluid HRM assay.

Body fluid	Gene	Amplicon size (base pairs)	Amplicon T _m		Primer sequence (5′–3′)
Body fluid	Gene	Amplicon size (base pairs)	°C	Range (± 3SD)	Primer sequence (5′–3′)
Saliva	HTN3	134	76.6	76.0 – 77.2	F: GCAAAGAGACATCATGGGTA R: GCCAGTCAAACCTCCATAATC
Vaginal	IL19	109	78.6	78.0 – 79.2	F: AACCACGGTCTCAGGAGATG R: TGACATTTGGGAAGGTGTCC
Menstrual blood	MMP10	178	81.9	81.5 – 82.3	F: GGGGGTGACGTTGGTCACTTCAGCTC R: GGGGGCTGGAGAATGTGAGTGGAGT
Semen	TGM4	164	82.7	82.4 – 83.0	F: ATGGTGTAAAGAGGACATGGTT R: GGGAAATGCAGCAGTCCAG
Skin	CCL27	142	84.9	84.8 – 84.9	F: AGCACTGCCTGCTGTACTCA R: AGATGCTGCGTTGAGCCA
Blood	ALAS2	222	86.8	86.6 – 86.9	F: TGTGTCCGTCTGGTGTAGTA R: GAGTCATTGGCAACAAAGCA
DNA	--		80.6	80.2 – 80.9	NA (product originates from IL19)

*underline indicates non-template sequence addition; report amplicon size includes the non-template sequence additions