Figure 6. Intrathecal lumbar administration of AS induces significant down-regulation of the CaV3.2 channel protein expression in lumbar DRGs of STZ rats.
Protein expression of CaV3.2 channel was analyzed and expressed as a ratio to that of the housekeeping protein β-actin. The bar graph shows that in AS-CaV3.2-treated rats (closed bar) we detected, on average, about a 60% decrease in CaV3.2 channel expression as compared with that of the MIS-control group (open bar; *, p<0.05). The protein measurement was performed 10 hours after the last AS-CaV3.2 injection. (n = 5 rats in MIS-control group; n = 4 rats in AS group). The insert illustrates original immunoblots from the representative experiments. (Since there was no difference in protein expression of CaV3.2 channel in STZ rats treated with either MIS or saline, the findings are compiled and presented as MIS-Control).