Figure 5. Chromatin immunoprecipitation (ChIP) indicates that Myb2R4 binds the Myb2R1 promoter.
A, Verification of expression of FLAG-tagged Myb2R4 in P. infestans transformant T10 by RT-PCR (left) and immunoblot analysis (right). B, ChIP analysis using the FLAG-tagged strain. Complexes from cross-linked chromatin were purified using anti-FLAG or control IgG, and subjected to qPCR using primers targeted to 250-nt promoter regions of the indicated genes.