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. 2014 Apr 4;9(4):e92086. doi: 10.1371/journal.pone.0092086

Figure 6. Silencing Myb2R3.

Figure 6

A, Characteristics of six transformants (T1 to T6) and wild-type (WT). Top panel shows levels of Myb2R3 mRNA in sporulating hyphae, indicating that T2, T5, and T6 show the strongest silencing. Lower panel denotes Myb2R3 copy number, based on qPCR using primers detecting both the native gene and transgene. Similar results were obtained using primers for the Ste20 intron that is between the sense and antisense regions of the hairpin construct (not shown). B, Sporulation and growth rates of transformants after 8 days in cultures exposed to a 12 hr light/dark cycle or continuous darkness. T2, T5, and T6 are the silenced transformants, and EV is a control transformant obtained using empty vector. C, Sporulation of wild-type and silenced transformants on tomato leaflets exposed to a 12 hr light/dark cycle.