Skip to main content
. 2014 Apr 4;9(4):e92086. doi: 10.1371/journal.pone.0092086

Figure 7. Over-expression of Myb genes.

Figure 7

A, Growth of transformants expressing FLAG-Myb constructs. Values are normalized to empty vector (EV) controls; asterisks mark values significantly different from controls, and error bars reflect standard deviation between multiple transformants. B, Example of sectoring in a transformant over-expressing Myb2R4, with the arrow indicating a zone of more rapid growth. C, Loss of expression of Myb2R4 transgene. Indicated are two transformants (T10, T11) and derivatives which lost or reduced expression (T10-R and T11-R, respectively). D, Myb2R4 mRNA levels in hyphae (top panel, normalized to wild-type) and transgene copy number (lower panel) in the strains from panel C. E, Sporulation rate on leaves and rye-sucrose agar in the strains from panel C. F, Transformant over-expressing Myb2R1 (T20) and a derivative (T20-R) with reduced transgene expression level and copy number.