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. 2014 Apr 4;9(4):e92086. doi: 10.1371/journal.pone.0092086

Figure 9. Serial sporulation induced by over-expression of Myb3R6.

Figure 9

A, mRNA levels of Myb3R6 in wild-type, using the same developmental stages as in Fig. 2. Error bars represent standard deviation from three replicates. B, mRNA levels in three over-expressing transformants (T60, T61, T62) and wild-type (WT). C, inferred stages of aberrant germination observed in sporangia harvesting from rye-sucrose agar cultures. Images show sporangium of normal appearance (panel 1), sporangium extending germ tube (panel 2), secondary sporulation (panels 3,4), and formation of a third (panel 5) and fourth sporangium (panel 6). D, Percent of sporangia exhibiting serial sporulation from 8-day cultures of strains expressing Myb3R6::FLAG (T60, T61, T62), a non-expressing revertant of T61 (T61-R), an empty vector transformant (EV), and strains expressing Myb2R5::FLAG (T50, T51, T52). E, Age-dependence of serial sporulation. Data are from a Myb3R6::FLAG transformant, a Myb2R5::FLAG transformant, and an empty vector transformant.