Figure 6. ADP and TxA₂ play significant roles in echicetin bead induced platelet aggregation.

Washed human platelets were preincubated with P2Y₁₂ antagonist (AR-C69931; 0.1 μM, 5 min), P2Y₁ antagonist (MRS2179; 1 μM, 5 min) or TxA₂ receptor antagonist (SQ-29548; 1 μM, 5 min) before EP0.1 or vWF/R stimulation. Representative aggregation curves of platelets stimulated with EP0.1 (A) or vWF/R (B) are shown. C, D. The graphs shows corresponding average values of aggregation from A and B. Platelets stimulated with EP0.1 (E) or vWF/R (F) were lysed on indicated time and the phosphorylation of PKB, ERK and p38 was analyzed by Western blot. Representative blots of three independent experiments are shown.⁺p<0.05 versus EP0.1.