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. 2014 Apr 4;9(4):e93699. doi: 10.1371/journal.pone.0093699

Figure 4. CD36 expression on MDM cells cultivated in HEMA condition in presence of the LPS inhibitor polymyxin B.

Figure 4

PBMCs were cultivated in HEMA condition for three days followed by additional three days in presence of 50/mL rNef/myr, 100 ng/mL LPS or 10 μg/ml polymyxin B. In some cultures, polymyxin B was added 15 min before the Nef and LPS treatment. (A) Representative histogram of MDMs analyzed by flow cytometry for the expression of CD36. The histogram on the right shows the respective treatment with LPS, polymyxin B and polymyxin B+LPS. The fluorescence intensities were compared to untreated cells (Ctr). In (B) similar analysis by replacing LPS with rNef/myr is shown. Both in (A) and (B) matched isotypes (Isotype) were used as control of non-specific fluorescence signals. SYTOX Blue was used to exclude dead cells. The results are representative of three independent experiments.