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. 2014 Apr 4;9(4):e93658. doi: 10.1371/journal.pone.0093658

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© 2014 Rodriguez et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Lane 1: Molecular weight markers (100-pb ladder). Lane 2: DNA from Angiostrongylus costaricensis (positive control). Lane 3: Negative control. Lanes 4–7: Presumptive cases with respective DNA concentrations: 6.9 μg/mL; 7.52 μg/mL; 2.3 μg/mL; 2.1 μg/mL. Lane 8: Mix (reaction control for detection of possible contaminants).