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. 2014 Apr 4;9(4):e93685. doi: 10.1371/journal.pone.0093685

Figure 2. HPLC chromatograms at 254 nm and MS spectra of liquid cultures of A. fumigatus after seven days of incubation.

Figure 2

A: Overlaid chromatograms for the control culture (below) and cultures exposed to 5 μg PG/ml (above). The grey box indicates the gliotoxin peaks. The PG-culture chromatogram is almost identical to that of the control and those for cultures containing LTS and LPS, with the exception of the intensity of the gliotoxin peak at 19.5 minutes. This indicates that the presence of PAMPs did not induce the production of any new metabolites or in any other way radically altered the metabolic profile. B: A representative MS spectrum showing the ion pattern of gliotoxin, which features prominent ions at 263.1, 245.1 and 227.1, m/z together with various background ions. The mother ion at 327.4 m/z is also indicated.