Table 2. Primers used for strand-specific RT-PCR in this study.
| Transcript to be detected | Sequence (5′––>3′) | RT-PCR* | Direction | cDNA (bp)** |
| CXCL1 mRNA | GCCAAGCCACAGGGGCGCCCGT | PCR | Forward | 231 |
| ACTTGGGGACACCCTTTAGCATC | PCR | Reverse | ||
| GAPDH mRNA | CCCATCACCATCTTCCAGGAGCGAG | PCR | Forward | 285 |
| GTTGTCATGGATGACCTTGGCCAGG | PCR | Reverse | ||
| IL-23A mRNA | CAAGGACAACAGCCAGTTCTGTT | PCR | Forward | 176 |
| GGTGATCCTCTGGCTGGAGGAGC | PCR | Reverse | ||
| iNOS mRNA | CCAACCTGCAGGTCTTCGATG | PCR | Forward | 257 |
| GTCGATGCACAACTGGGTGAAC | PCR | Reverse | ||
| iNOS asRNA# | TGCCCCTCCCCCACATTCTCT | RT | Forward | |
| ACCAGGAGGCGCCATCCCGCTGC | PCR | Forward | 185 | |
| CTTGATCAAACACTCATTTTATTAAA | PCR | Reverse | ||
| NF-κB p65 mRNA | ACCCCTTTCAAGTTCCCATAGA | PCR | Forward | 262 |
| ACCTCAATGTCTTCTTTCTGCAC | PCR | Reverse | ||
| NF-κB p50 mRNA | CCTGCTCCTGGAGGGTGACGCC | PCR | Forward | 254 |
| GTATGTCAAATACCTGCCAGTTG | PCR | Reverse | ||
| TNF-α mRNA | TCCCAACAAGGAGGAGAAGTTCC | PCR | Forward | 275 |
| GGCAGCCTTGTCCCTTGAAGAGA | PCR | Reverse |
*The oligo(dT) primer was used for reverse transcription (RT) to synthesize the complementary DNAs (cDNAs) for each mRNA.
**The size of the cDNA fragment amplified by each pair of polymerase chain reaction (PCR) primers is shown in base pairs (bp).
#
A sense primer was used for the reverse transcription of the iNOS antisense transcript (asRNA).
CXCL1, chemokine (C-X-C motif) ligand 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-23A, interleukin 23, α subunit p19; iNOS, inducible nitric oxide synthase; NF-κB, nuclear factor κB; TNF-α, tumor necrosis factor α.