Figure 1.

TMEM127 mutations in renal cell carcinoma. (A) Distribution of the TMEM127 mutations found in four RCCs along the TMEM127 protein structure; (B) main clinical and genetic features of these patients; (C) real-time PCR quantification of two TMEM127-mutant and four non-mutant RCC tumors. Each sample was run in triplicate in two separate experiments. Results were combined for each group and are presented as mean ± SEM; (D) pattern of intracellular distribution of four TMEM127 mutants and WT GFP-fusion constructs transfected in HeLa cells shown as the fraction of diffuse cytoplasmic localization by confocal microscopy from at least five independent experiments and an average of 220 cells counted per construct (*P < 0.05).