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. 2013 Dec 13;23(9):2428–2439. doi: 10.1093/hmg/ddt638

Figure 4.

Figure 4.

The enlarged vesicle phenotype of Rab5 activation is reduced by TMEM127 depletion or absence. (A) HeLa cells expressing two independent TMEM127 siRNAs (#2 and #4) or a control siRNA (si-ctl) were transfected with Flag-tagged Rab5Q79L and fixed after 24 h for staining and confocal microscopy. Flag-Rab5Q79L mutant is shown in red and DAPI-stained nuclei are in blue. (B) Rab5Q79L staining of HeLa cells expressing siTMEM127#2 without (left) or after transfection of a TMEM127 construct engineered to be resistant to siRNA#2 (WT-TMEM127SR). (C) MEFs from Tmem127−/− or Tmem127+/+ embryos were transfected with Rab5Q79L and a GFP empty vector (left), GFP-WT TMEM127 (middle) or GFP-Ser147del TMEM127 (right). (D) Quantification of the number of enlarged vesicles per cell of each cell type shown in (C) is indicated. Data are representative from at least three independent experiments (P < 0.05; NS = non-significant). Scale bars are 10 mm. Data are representative from at least three independent experiments (P < 0.05; NS = non-significant). Scale bars are 10 µm.