Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 20;23(9):2468–2480. doi: 10.1093/hmg/ddt642

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Characterization of the cytoplasmic vesicles observed in affected male mice. (A–F) Dual immuno-staining for amelogenin (red fluorescence) or (G–I) ameloblastin (red fluorescence) and components of the secretory pathway (green fluorescence) in affected male mice (n = 4) secretory ameloblasts indicate arrest of amelogenin trafficking at a stage prior to the trans-Golgi. (A) The ER membrane protein calnexin circumscribes the amelogenin-containing vesicles (arrows), while the ER luminal molecule protein disulphide isomerase (B) co-localizes with amelogenin within the vesicles (arrows). (C) Immuno-reactivity for the ER/Golgi intermediate compartment molecule Ergic 53 also co-localizes with amelogenin in the vesicles (arrows), whereas (D) β-COP, a component of COP1 vesicles found at the cis-Golgi and ER/Golgi intermediate compartment, co-localizes with amelogenin at the periphery of the vesicles (arrows). (E) The cis-Golgi protein GM130 co-localizes with amelogenin within the vesicles (arrows). (F) Golgin 245, a trans-Golgi protein, showed a variable pattern of immuno-reactivity; in most instances, little amelogenin co-localization was observed in the vesicles (white arrows), although, less typically, partial or complete co-localization was seen (green arrows). (G) Dual immunofluorescence for ameloblastin and the ER membrane protein calnexin indicates that calnexin circumscribes the inclusions (arrows). (H) Similar analyses using antibodies directed against ameloblastin and the ER luminal molecule protein disulphide isomerase (PDI) indicate co-localization within the vesicles (arrows). (I) In contrast, dual immunofluorescence with ameloblastin and Ergic 53, a protein found in the ER/Golgi intermediate compartment, demonstrates distinct localization within the ameloblasts (arrows). Nuclei are stained with DAPI (blue). Scale bars: 5 μm.